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Cet abstract a été assigné à session OB Free papers: Medical retina
TitreThe effect of upregulated LOX and LOXL2 on inflammation and fibrosis in a laser induced CNV model.
Abstract Nr.243
ButLysyl oxidase (LOX) and lysyl oxidase-like protein 2 (LOXL2) are involved in the cross-linking of collagen and elastin. Therefore, these proteins play a major role in the process of fibrosis. This study was designed to elucidate the role of LOX and LOXL2 in inflammation and fibrosis after choroidal neovascularization (CNV).
MéthodesCNV was induced in 8 to 10 weeks old C75Bl/6 mice, by placing 3 laser spots (50µm, 0.05 s and 400mW). Mice were sacrificed 2, 4, 7, 14, 28 and 35 days after lasering. LOX and LOXL2 expression in choroid and retina was analyzed by using quantitative real time RT-PCR. Inflammation was studied by an immunohistochemical staining for CD45; fibrosis was evaluated by a Sirius Red and Trichrome staining.
RésultatsBoth LOX and LOXL2 expression were significantly increased over time in the choroid and retina of lasered mice compared to the expression of LOX in non-lasered eyes. On day 2 and 4 after laser, the number of inflammatory cells was increased by 16% compared to control. Both Sirius Red and Thrichrome staining showed a significant increase of collagen deposition in the choroid on day 14 (43%), day 28 (44%) and day 35 (40%) after laser compared to non-lasered eyes.
ConclusionA biphasic upregulation of LOX and LOXL2 was observed after CNV induction, which was associated with the inflammatory and fibrotic phase, respectively. Our data suggest that LOX and LOXL2 play a role in the process of inflammation and fibrosis after the induction of choroidal neovascularization. This finding can open new perspectives in the treatment of age related macular degeneration by inhibiting LOX and LOXL.
Auteur 1
NomVAN DE VEIRE
InitialesS
InstitutKUL
VilleLeuven
Auteur 2
NomVan Bergen
InitialesT
InstitutKUL
VilleLeuven
Auteur 3
NomVandewalle
InitialesE
InstitutKUL
VilleLeuven
Auteur 4
NomMoons
InitialesL
InstitutKUL
VilleLeuven
Auteur 5
NomHerman
InitialesJean
InstitutKUL
VilleLeuven
Auteur 6
NomSmith
InitialesV
InstitutArresto Biosciences
VillePalo Alto
Auteur 7
NomOgg
InitialesS
InstitutArresto Biosciences
VillePalo Alto
Auteur 8
NomStalmans
InitialesI
InstitutKUL
VilleLeuven
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