Titel | FRO: Study of the role of P2Y6 receptors in the development of experimental autoimmune uveitis |
Abstract Nr. | 246 |
Doel | During autoimmune uveitis, auto-reactive T lymphocytes recognize retinal antigens, penetrate the eye and start an inflammatory reaction. The mechanisms responsible for this pathological T lymphocyte activation are poorly understood. Nucleotides are intracellular mediator and normally present in a very low concentration in the extracellular media. However, during inflammation, nucleotides are released from the cells and can act as a danger signal through P2 receptors activation. To study the potential role of P2Y2 receptors in the development of experimental autoimmune uveitis (EAU). |
Methodes | EAU were induced in WT and P2Y2 KO mice receptors by IRBP 1-20 peptide immunisation. Twelve days later, T lymphocytes from spleen and lymph nodes were semi-purified and restimulated by IRBP 1-20 peptide. Lymphocyte proliferation was measured by thymidine incorporation and cytokines secretion by ELISA. EAU development after adoptive transfer of activated lymphocytes was followed through fundus examination of the eyes and VCAM-1 retinal expression analysed by immunofluorescence. |
Resultaten | Autoreactive lymphocytes generated in KO mice proliferate less and produce less IFNγ, IL-17, IL-6 and MIP2α after IRBP 1-20 peptide restimulation than lymphocytes generated in WT mice. Following adoptive transfer, KO lymphocytes induced less disease as assessed by clinical grading and VCAM1 expression. |
Conclusie | Our data shows that P2Y2 KO mice are less likely to develop an immune response against a retinal antigen than WT. This suggests that P2Y2 receptor might act as danger receptor during EAU development. |
Naam | JUDICE DE MENEZES RELVAS |
Initialen | L |
Stad | Brussels |