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This abstract is assigned to session BIO: Belgian Immuno Ophthalmology Club
Presentation typeOB - oral
TitleFRO: Study of the role of P2Y receptors in the development of experimental autoimmune uveitis
PurposeTo study the potential role of P2Y2 receptors in the development of experimental autoimmune uveitis (EAU).
MethodsEAU were induced in WT and P2Y2 KO mice receptors by IRBP 1-20 peptide immunisation. Twelve days later, T lymphocytes from spleen and lymph nodes were semi-purified and restimulated by IRBP 1-20 peptide. Lymphocyte proliferation was measured by thymidine incorporation, cytokines secretion by ELISA and surface molecules are detected by FACS. EAU development after adoptive transfer of activated lymphocytes was followed through fundus examination of the eyes and VCAM-1 retinal expression analysed by immunofluorescence.
ResultsAutoreactive lymphocytes generated in KO mice proliferate less and produce less pro-inflammatory cytokines after IRBP 1-20 peptide restimulation than lymphocytes generated in WT mice. Following adoptive transfer, KO lymphocytes induced less disease as assessed by clinical grading and VCAM1 expression.
ConclusionOur data shows that P2Y2 KO mice are less likely to develop an immune response against a retinal antigen than WT. This suggests that P2Y2 receptor might act as danger receptor during EAU development.
Author 1
Last nameJUDICE DE MENEZES RELVAS
InitialsL
DepartmentCHU St Pierre, CHU Brugmann and IRIBHM
CityBrussels
Author 2
Last nameWillermain
InitialsF
DepartmentCHU St Pierre, CHU Brugmann and IRIBHM
CityBrussels
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