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This abstract is assigned to session Poster session in O'Bistro

Title

The effect of AMA0076, a locally acting rho kinase (ROCK) inhibitor, on IOP in Dutch Belted Rabbits

Purpose	To determine the intra ocular pressure (IOP) lowering efficacy of the local ROCK inhibitor, AMA0076, in Dutch Belted rabbits.
Methods	Dutch Belted rabbits (5 rabbits/group) received topically a single dose of AMA0076 in one eye. A concentration range of 0.031 - 0.625% AMA0076 was tested. The contralateral eye served as control and was treated with saline. IOP was measured at baseline and 30 min, 1, 2, 3, 4, 5, 6, 7, 8h post topical administration.
Results	Single topical administration of AMA0076 significantly lowered IOP in all groups compared to the control eye (p<0.05). Mean baseline IOP during the experiments was between 20.6 and 21.5 mmHg. Thirty minutes after topical treatment, a concentration dependent reduction in IOP was demonstrated with a ΔIOP ranging from 3.9mmHg – 7mmHg compared to the control eye. Maximal IOP reduction of each concentration following single dose administration of AMA0076 was observed ±2 hours after instillation with a ΔIOP ranging from 4.6mmHg and 7.5mmHg. The IOP lowering effect with the highest concentrations of AMA0076 (0.625% and 0.125%) was sustained during the experiment.
Conclusion	AMA0076 was effective in rapidly lowering IOP in a dose depended and sustained manner after a single topical dose in Dutch Belted rabbits. This new class of local ROCK inhibitors has potential therapeutic value for the IOP lowering treatment of glaucoma.

Authors 1

Last name	VAN DE VELDE
Initials	S
Department	Universiteit Leuven
City	Leuven

Authors 2

Last name	Van Bergen
Initials	T
Department	Universiteit Leuven
City	Leuven

Authors 3

Last name	Sijnave
Initials	D
Department	Universiteit Leuven
City	Leuven

Authors 4

Last name	Hollanders
Initials	K
Department	Universiteit Leuven
City	Leuven

Authors 5

Last name	Vandewalle
Initials	E
Department	Universiteit Leuven
City	Leuven

Authors 6

Last name	Moons
Initials	L
Department	Universiteit Leuven
City	Leuven

Authors 7

Last name	Leysen
Initials	D
Department	Amakem NV
City	Diepenbeek

Authors 8

Last name	Stalmans
Initials	I
Department	Universiteit Leuven
City	Leuven