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Title	24h IOP control by subchronic AMA0076 in Dutch Belted Rabbits
Abstract Nr.	P121

Purpose	To investigate IOP lowering efficacy of rho kinase (ROCK) inhibitors after subchronic treatment.
Methods	DBR (5 rabbits/concentration) were treated daily with a single topical dose of AMA0076 or Y-39983 in a masked fashion for 14 days. Saline was used as control in the contralateral eye. IOP was measured before, 3 and 7 hours after treatment. AMA0076 is a locally acting ROCK inhibitor, developed by Amakem Ophthalmics, to reduce side effects such as hyperemia.
Results	Mean baseline IOP before start of experiment was 21.0 ± 0.4 and 20.7 ± 0.4 mmHg for AMA0076 and Y-39983, respectively. Significant IOP reduction was observed in all treatment groups. A maximal IOP reduction of 39 and 35% was reached with AMA0076 0.1% and Y-39983 0.05%, respectively. Initially, IOP returned to baseline values 24h after administration of AMA0076. After 3 days of treatment with AMA0076 a sustained IOP lowering effect was present: IOP did not return to baseline values, but remained significantly lower for all concentrations of AMA0076. Treatment with Y-39983 did not show this sustained IOP decrease. As a result of this sustained IOP lowering effect smaller IOP fluctuations were observed in animals treated with AMA0076 compared to Y-39983. Hyperemia was observed with all concentrations of Y-39983 whereas only mild hyperemia was induced after administration of AMA0076 0.4.
Conclusion	Treatment of rabbits with AMA0076 resulted in IOP reduction that was more sustained and associated with smaller peak-through fluctuations than Y-39983.

Authors 1

Last name	VAN DE VELDE
Initials	S
Department	KUL Ophthalmo
City	leuven

Authors 2

Last name	Van Bergen
Initials	T
Department	KUL Ophthalmo
City	Leuven

Authors 3

Last name	Hollanders
Initials	K
Department	KUL Ophthalmo
City	Leuven

Authors 4

Last name	Sijnave
Initials	D
Department	KUL Ophthalmo
City	Leuven

Authors 5

Last name	Vandewalle
Initials	E
Department	KUL Ophthalmo
City	Leuven

Authors 6

Last name	Castermans
Initials	K
Department	Amakem Ophthalmics
City	Diepenbeek

Authors 7

Last name	Kindt
Initials	N
Department	Amakem Ophthalmics
City	Diepenbeek

Authors 8

Last name	Moons
Initials	L
Department	KUL Biologie
City	Leuven

Authors 9

Last name	Leysen
Department	Amakem Ophthalmics
City	Diepenbeek

Authors 10

Last name	Stalmans
Initials	I
Department	KUL Ophthalmo
City	Leuven