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TitreHistology of Vitreous Floaters, after removal by pars plana vitrectomy
ButTo investigate the histopathology of vitreous floaters.
MéthodesTen consecutive (10) patients with vitreous floaters creating visual disturbance and affecting their daily life underwent 23G vitrectomy. First, a core vitrectomy was performed. If not already present, a posterior vitreous detachment was created. Next, a shaving of the vitreous base was done. All the aspirated fluid was collected in the vitrectomy cassette and fixed in Cytorich Red. After fixation, the fluid was centrifugated and the pellet was embedded in paraffin and sectioned and stained routinely for microscopy with H&E and PAS. Immunostainings for lymphocytes (CD3, CD20), macrophages (CD68) and retinal (nse) and glial tissue (gfap) were performed.
RésultatsIn all cases sufficient material was collected for histology. The vitreous core consisted of fine fibrillar tissue in which floaters were recognised as a small fragment of glial tissue with cells with fibrillar cytoplasm, staining specifically for gfap. In most cases macrophages were randomly and diffuse present.
ConclusionVitreous floaters seem to originate from glial origin
Auteur 1
NomVAN GINDERDEUREN
InitialesR
InstitutOogziekten, UZLeuven
VilleLeuven
Auteur 2
NomSTALMANS
InitialesP
InstitutOogziekten, UZLeuven
VilleLeuven
Auteur 3
NomVAN CALSTER
InitialesJ
InstitutOogziekten, UZLeuven
VilleLeuven
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