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This abstract is assigned to session OB Poster session > in O'Bistro

Title

Histology of Vitreous Floaters, after removal by pars plana vitrectomy

Purpose	To investigate the histopathology of vitreous floaters.
Methods	Ten consecutive (10) patients with vitreous floaters creating visual disturbance and affecting their daily life underwent 23G vitrectomy. First, a core vitrectomy was performed. If not already present, a posterior vitreous detachment was created. Next, a shaving of the vitreous base was done. All the aspirated fluid was collected in the vitrectomy cassette and fixed in Cytorich Red. After fixation, the fluid was centrifugated and the pellet was embedded in paraffin and sectioned and stained routinely for microscopy with H&E and PAS. Immunostainings for lymphocytes (CD3, CD20), macrophages (CD68) and retinal (nse) and glial tissue (gfap) were performed.
Results	In all cases sufficient material was collected for histology. The vitreous core consisted of fine fibrillar tissue in which floaters were recognised as a small fragment of glial tissue with cells with fibrillar cytoplasm, staining specifically for gfap. In most cases macrophages were randomly and diffuse present.
Conclusion	Vitreous floaters seem to originate from glial origin

Author 1

Last name	VAN GINDERDEUREN
Initials	R
Department	Oogziekten, UZLeuven
City	Leuven

Author 2

Last name	STALMANS
Initials	P
Department	Oogziekten, UZLeuven
City	Leuven

Author 3

Last name	VAN CALSTER
Initials	J
Department	Oogziekten, UZLeuven
City	Leuven