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This abstract is assigned to session SB0-BOG Free paper: Glaucoma/Glaucome

Title	Haptoglobin knockout mice exhibit morhologic changes consistent with iridocorneal dysgenesis and glaucoma
Abstract Nr.	217

Purpose	To investigate the ocular phenotype of mice that lack the acute phase protein Haptoglobin (Hpko).
Methods	Hpko mice were clinically evaluated at 3, 8 and 16 weeks of age, eye pressure was measured at 3 and 8 weeks. Mice that showed macroscopic changes of the eyes were sacrificed and their eyes were enucleated, fixed in paraformaldehyde, histologically sectioned and stained with hematoxylin and eosin. The diameter of the eye was measured and the histomorphology was evaluated and compared with eyes of normal wild-type mice.
Results	Clinical observation revealed that between 10 and 20 % of the Hpko mice exhibited gradual opacification of the cornea and shrinkage of the eyes. These mice were investigated. The eyes of Hpko mice were between 10 and 40 % smaller than eyes of normal mice. The eyes of Hpko mice showed rudimentary and more widely spread processi of the ciliary body. Moreover the anterior chamber showed goniosynechia and irido-corneal adhesions, as well as attenuation the trabecular meshwork. The neuroretina showed a variable atrophy in ganglion cell layers in all all Hpko mice and in the inner nuclear and plexiform layer in 80 %.
Conclusion	Haptoglobin knockout mice have structural abnormalities of the anterior segment and neuroretina comparable to human iridocorneal dysgenesis and developmental glaucoma.

Author 1

Last name	VAN GINDERDEUREN
Initials	R
Department	Oogziekten UZ Leuven
City	Leuven

Author 2

Last name	Galicias-Rosas
Initials	G
Department	Immunology
City	Leuven

Author 3

Last name	Vandeveire
Initials	S
Department	Oogziekten
City	Leuven

Author 4

Last name	Van Bergen
Initials	T
Department	Oogziekten
City	Leuven

Author 5

Last name	Van Schelvergem
Initials	K
Department	Oogziekten
City	Leuven

Author 6

Last name	Ceuppens
Initials	J
Department	Immunology
City	Leuven

Author 7

Last name	Stalmans
Initials	I
Department	Oogziekten
City	Leuven