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Deze abstract is toegekend aan sessie SBO-BOG Free papers: Medische retina en oculaire inflammatie /Rétine médicale et inflammation oculaire
TitelFRO: Role of VEGF-isoforms in pathological angiogenesis
DoelThe aim of this project is to study the specific role of the VEGF-isoforms in pathological angiogenesis, and to investigate the effect of blocking a single isoform on formation of choroidal neovascularisation (CNV).
MethodesVEGF-isoform specific mice (VEGF 120/+, VEGF 164/164 and VEGF188/188 mice) , as well as double transgenic mice (VEGF 120/164, VEGF 164/188 and VEGF120/188 mice) are used to study the role of VEGF-isoforms in pathological angiogenesis. In these mice models pathological angiogenesis is induced. CNV is induced by placing 3 laser spots at 9, 12 and 3 o’clock position (50µm, 0.05 s and 400mW). The amount of the new formed blood vessels is determined by FITC-dextrane perfusion. A fluorescent analysis of the whole mount choroids gives a threedimensional image of the vascular organization, remodeling and differentiation of the blood vessels.
ResultatenThe VEGF-isoform specific mice (VEGF 120/120, VEGF 164/164 and VEGF188/188 mice) were backcrossed to a C75Bl/6 background to be able to induce CNV and study the differential role of a single isoform on pathological angiogenesis. Subsequently, these mice will be intercrossed to obtain double transgenic mice to study the effect of blocking a single isoform on CNV production. Preliminary data will be presented at the OB-meeting 2008.
ConclusieThis study will shed new light on the different role and the inhibition of the VEGF-isoforms in choroidal neovascularisation during age-related macular degeneration. Thus, our project may open new perspectives for the treatment of various retinopathies that are known to be associated with VEGF upregulation.
Auteur 1
NaamVAN BERGEN
InitialenT
StadLeuven
Auteur 2
NaamVAN DE VEIRE
InitialenS
Auteur 3
NaamMOONS
InitialenL
Auteur 4
NaamSTALMANS
InitialenI
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