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Deze abstract is toegekend aan sessie SBO-BOG Free papers: Miscellanous
TitelFRO: Cultivated Limbal Stem Cell Transplantation - A New Approach
DoelA variety of conditions including chemical or thermal burns, Steven Johnson’s Syndrome, Aniridia, advanced Ocular Cicatricial Pemphigoid, extensive microbial infections etc. may lead to Limbal stem cell deficiency (LSCD) in the eye. The signs of LSCD include vascularization of the cornea with persistent epithelial defects and haze, resulting in a considerable loss of visual acuity. Here we take a look at cultivated limbal stem cell transplantation as new treatment modality to restore functioning limbal stem cells and improve outcome of any subsequently required keratoplasties.
MethodesLimbal explants taken from cadaveric donor corneas in case of bilateral disease or from the contralateral good eye in unilateral disease are placed in culture on amniotic membrane that has been denuded of its epithelium and spongy layer. The limbal stem cells are allowed to grow out of the explant and onto the amniotic basement membrane over a period of 2 to 3 weeks. The culture medium used is progenitor cell targeted, cholera toxin free as well as free from any animal derived products. The cultured cells along with the amniotic membrane are transplanted using an innovative technique which is simple, quick and requires minimal manipulation of the delicate stem cell graft.
ResultatenTwo patients have been transplanted with this new technique: one received an allogenic graft grown from a cadaveric donor whilst the second received a graft generated from a biopsy taken from the contralateral good eye. Early post operative results show that the graft is securely in place and free from any signs of inflammation or rejection. Further follow up is required to establish long term outcome.
ConclusieAlthough cultivated limbal stem cell transplantation has been in practice for some time now, an animal free culture system using processed, standardized amniotic membrane has yet to be described in a clinical setting. The new surgical technique described herein allows for secure graft placement without the need for extensive graft manipulation or sutures.
Auteur 1
NaamZAKARIA
InitialenN
StadAntwerp
Auteur 2
NaamKOPPEN
InitialenC
Auteur 3
NaamVAN TENDELOO
Auteur 4
NaamBERNEMAN
InitialenZ
Auteur 5
NaamTASSIGNON
InitialenMJ
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