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TitelFRO: Anti-PLGF, a good alternative to anti-VEGF in the treatment of AMD?
Abstract Nr.244
DoelTo complete the efficacy study of PlGF blockage in inhibiting choroidal neovascularization (CNV) in a mouse model of AMD.
MethodesA laser-induced CNV model was used in C57Bl/6 mice. Mice were injected intraperitoneally with 6.25; 12.5 or 25 mg/kg of an anti-PlGF-antibody, an anti-VEGF receptor-2 (VEGFR2) antibody, a combination of both, or a control antibody. The CNV lesions were evaluated on flat mounts and serial sections after 14 days, by immunostaining for endothelial, inflammatory and smooth muscle cells cells (CD31, F4/80 and alfa-SMA respectively). Presence of edema was checked by measuring the BC ratio. Combination therapy of anti-PlGF and anti-VEGFR2 to lower the number of intravitreal injections was done.
ResultatenAnti-PlGF or anti-VEGFR2 comparably inhibited CNV by ± 50%. Moreover, a combination treatment of the optimal dose of anti-PlGF and a lower dose of anti-VEGFR2 further suppressed CNV to ± 70%, allowing reducing the dose of anti-VEGFR2 by fourfold. Edema was lowered in eyes treated with anti-PlGF whereas not with anti-VEGFR2. Combination therapy of both antibodies led to not only lower dosing of anti-VEGFR2 but also lower number of injections.
ConclusieAnti-PlGF treatment inhibits CNV formation in a mouse model of AMD, and enhances the efficacy of anti-VEGFR-2, allowing the dose and amount of anti-VEGF therapy to be lowered and the potential adverse effects to be minimized. Moreover and contrary to anti-VEGFR2, anti-PlGF lowers the invalidating edema present in CNV.
Auteur 1
NaamVAN DE VEIRE
InitialenS
InstituutKUL
StadLeuven
Auteur 2
NaamVinores
InitialenS
InstituutJohn Hopkins
StadBaltimore
Auteur 3
NaamVan Bergen
InitialenT
InstituutKUL
StadLeuven
Auteur 4
NaamMazzone
InitialenM
InstituutKUL
StadLeuven
Auteur 5
NaamMoons
InitialenL
InstituutKUL
StadLeuven
Auteur 6
NaamCarmeliet
InitialenP
InstituutKUL
StadLeuven
Auteur 7
NaamStalmans
InitialenI
InstituutKUL
StadLeuven
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