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Titel	Lack of monosomy chromosome 3 in conjunctival melanoma and naevi
Abstract Nr.	132

Doel	Monosomy of chromosome 3 has been established as an important prognostic factor for intraocular tumors, and is being used in practice. This study investigates monosomy 3 in conjunctival melanoma and naevi.
Methodes	Paraffin embedded sections of 13 conjunctival melanomas, 11 conjunctival naevi and three samples of primary acquired melanosis (PAM) were investigated by fluorescence in situ hybridization (FISH) using a DNA-specific probe for the centromere region of chromosome 3. In addition, monosomy 3 was further assessed with FISH on nuclei, isolated from paraffin-embedded tissue and compared to the results of FISH on regular histological paraffin sections.
Resultaten	Combining FISH on histological paraffin sections and on isolated nuclei showed no monosomy 3 in conjunctival melanoma or naevi. Of 13 conjunctival melanomas one sample could not be evaluated with FISH on whole sections, but satisfactory results were obtained on nuclear preparations. Although five melanoma samples seemed to show monosomy 3 when FISH was applied on sections, this was not confirmed on isolated nuclei preparations.
Conclusie	In difference with uveal melanoma where monosomy 3 is present in up to 60% of samples, conjunctival naevi and melanoma do not show monsomy 3. The high false-positive rate obtained with FISH on histological paraffin sections determines the necessity of using nuclear suspensions for monosomy studies.

Auteur 1

Naam	MISSOTTEN
Initialen	GS
Instituut	Leiden University Medical Center, Nederland & Catholic University Leuven, België
Stad	Leiden - Leuven

Auteur 2

Naam	Maat
Initialen	W
Instituut	Leiden University Medical Center, Nederland
Stad	Leiden

Auteur 3

Naam	Jordanova
Initialen	JS
Instituut	Leiden University Medical Center, Nederland
Stad	Leiden

Auteur 4

Naam	De Keizer
Initialen	RJ
Instituut	Leiden University Medical Center, Nederland & Antwerp University, België
Stad	Leiden - Antwerpen