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Cet abstract a été assigné à session AOB Poster session1
TitreEarly results of a multicenter clinical trial: Regenerating the anterior cornea through standardized transplantation of limbal epithelial stem cells
Abstract Nr.1026
ButTo report the early results of transplanting non-xenogenic, bioengineerd, composite grafts of cultured limbal epithelial cells on standardized amniotic membranes in patients with limbal stem cell deficiency, in a phase II multicenter clinical trial.
MéthodesSo far a total of 6 patients with severe limbal stem cell deficiency were transplanted in this phase II multicenter trial. All of the 6 patients suffered from unilateral, total limbal stem cell deficiency.
The limbo-amnion grafts were generated by cultivating limbal epithelial stem cells from the contralateral donor eye on a standardized amniotic membrane. The cells were cultured for a period of 2 weeks in a xenogenic-free culture medium and the composite graft was transplanted with a standardized 'no touch' surgical technique.
RésultatsThe mean follow-up of the 6 patients so far is 10 weeks (range 1-19 weeks). Three patients had an improvement in pain and 2 patients reported a slight improvement in photophobia. Two of the 6 patients had a gain in visual acuity from hand movements to finger counting.
ConclusionIn this limited number of patients a functional improvement was achieved. So far, the grafts were well-tolerated, and not associated with side effects. The larger sample size and inclusion of allogenic transplant recipients in this phase II trial will further help ascertain safety and efficacy of a standardized transplantation of limbal epithelial stem cells.
Auteur 1
NomBEHAEGEL
InitialesJ
InstitutDept of Ophthalmology, UZB
VilleBrussels
Auteur 2
NomNi Dhubhghaill
InitialesS
InstitutDept of Ophthalmology, UZA
VilleAntwerp
Auteur 3
NomKoppen
InitialesC
InstitutDept of Ophthalmology, UZA
VilleAntwerp
Auteur 4
NomLeysen
InitialesI
InstitutDept of Ophthalmology, UZA
VilleAntwerp
Auteur 5
NomTen Tusscher
InitialesM
InstitutDept of Ophthalmology, UZB
VilleBrussels
Auteur 6
NomTassignon
InitialesMJ
InstitutDept of Ophthalmology, UZA
VilleAntwerp
Auteur 7
NomZakaria
InitialesN
InstitutCenter for Cell Therapy and Regenerative Medicine, UZA
VilleAntwerp
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