THE BELGIAN OPHTHALMIC ASSOCIATIONS
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This abstract is assigned to session SBO-BOG Posters session >> in Poster area - Foyer +1
| Title | Morphologic changes in Haptoglobin knockout mice, typical for anterior segment dysgenesis, are linked to diminished expression of C-Maf |
| Purpose | To study the ocular phenotype of mice that lack Haptoglobin (Hpko) and investigate whether these changes correlate with the expression of C-maf. C-maf is known to be expressed in embryonic life and to be involved in the development of the lens and anterior segment. |
| Methods | Hpko mice and wild type(WT)littermates were histological investigated at embryonic day(E)12,15 and 18 and postnatal day(P)1,4 and 21. The expression of C-maf was studied at mRNA level by rt-PCR. C-maf expression in eyes was compared in adult mice between clinically blind versus not-blind Hpko and WT mice and in embryos at E12,between offspring from clinical blind versus not-blind Hpko mothers. |
| Results | Eyes of Hpko mice were histological normal at E12 compared with WT mice. At P1 eyes of Hpko mice were significantly smaller (mean 29%); in 55% of mice the lens was not yet separated form the cornea. At P21 12% of the eyes from Hpko mice still showed contact between lens and cornea; anterior senychiae were present in 42% of the eyes, whereas these structural abnormalities were never observed in WT eyes. The ocular C-maf mRNA expression at E12 was significantly lower in Hpko embryos as compared to WT embryos (P<0.05). C-maf expression levels in eyes of Hpko non-blind mothers were intermediate |
| Conclusion | Haptoglobin knockout mice exhibit developmental disorders that are similar to those observed in developmental glaucoma. There is a delay in development of the anterior segment structures, which start during late embryonic life and continue after birth. C-maf expression is diminished at E12 and this may be linked to the aberrant anterior chamber development. |
Author 1
| Last name | VAN GINDERDEUREN |
| Initials | R |
| Department | Oogziekten |
| City | Leuven |
Author 2
| Last name | GALISIAS-ROSAS |
| Initials | G |
| Department | immunologie |
| City | Leuven |
Author 3
| Last name | Vandeveire |
| Initials | S |
| Department | Oogziekten |
| City | Leuven |
Author 4
| Last name | Van Bergen |
| Initials | T |
| Department | oogziekten |
| City | Leuven |
Author 5
| Last name | Ceuppens |
| Initials | J |
| Department | immunologie |
| City | Leuven |
Author 6
| Last name | Stalmans |
| Initials | I |
| Department | oogziekten |
| City | Leuven |