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Title

Tintelnotia destructans as an emerging opportunistic pathogen: First case of T. destructans superinfection in herpetic keratitis

Purpose	To present the first case of Tintelnotia destructans keratomycosis as a superinfection in herpetic keratitis.
Methods	Case report and literature review.
Results	We present a case of a 53-year-old woman who presented with a unilateral keratitis since 3 weeks without history of trauma or contact lens wear, not responding to topical ofloxacin. Polymerase Chain Reaction (PCR) of the corneal ulcer was positive for Herpes Simplex Virus type 1 (HSV-1). Signs and symptoms progressively improved after starting topical and systemic antiviral therapy. Six weeks later however, our patient presented with a new white infiltrate in the previous herpetic epithelial defect. In vivo confocal microscopy showed fungal hyphae and culture from corneal scrapings identified a hyphomycete. Intensive antimycotic therapy could not prevent a corneal perforation 1 week later. Penetrating keratoplasty was performed with intracameral injection of amphotericin B. Culture of the corneal button and PCR and sequence analysis on the fungal isolate confirmed the diagnosis of T. destructans keratomycosis. Six months after penetrating keratoplasty, biomicroscopy showed a clear graft without recurrence of fungal activity.
Conclusion	T. destructans is an emerging opportunistic pathogen causing severe keratomycosis. Despite intensive antimycotic therapy, rapid progression to corneal perforation can be seen. Early diagnosis using confocal microscopy, fungal culture and PCR can allow prompt initiation of treatment, which should be guided by in vitro susceptibility testing.
Conflict of interest	No

Authors 1

Last name	ROELS
Initials	D
Department	Dpt of Ophthalmology, Ghent University Hospital
City	Ghent, Belgium

Authors 2

Last name	COOREVITS
Initials	L
Department	Dpt of Laboratory Medicine, Ghent University Hospital
City	Ghent, Belgium

Authors 3

Last name	LAGROU
Initials	K
Department	National Reference Centre for Mycosis, Dpt of Laboratory Medicine, University Hospitals Leuven
City	Leuven, Belgium